Journal: bioRxiv

Article Title: Neural responses prior to licking onset in the striatal matrix compartment in mice

doi: 10.1101/2025.05.23.655747

Figure Lengend Snippet: A. Schematic illustration of the behavioral apparatus. The head and body of the mouse were restricted by a metal frame and tube. The two spouts were placed to the left and right of the mouth. Spout-licking behaviors were monitored using an infrared sensor. An optical fiber was connected to the optical probe implanted in the dorsomedial striatum (DMS) for fiber photometry of GCaMP6f. B. Diagram of an operant conditioning task. Each trial began when a light-emitting diode (LED) was illuminated. In the ipsilateral and contralateral blocks, a drop of sucrose water was delivered immediately after the mice licked a waterspout placed on the ipsilateral or contralateral side, respectively, of the fiber-implanted hemisphere. After water delivery, the LED was turned off, followed by an inter-trial interval (ITI). C. Schematic illustration of the measurement of GCaMP fluorescence from matrix neurons. GCaMP6f was selectively expressed in matrix neurons via injection of AAV5.CAG.Flex.GCaMP6f into the DMS of Calb1-IRES-Cre mice. An optical probe was implanted in the DMS to measure the calcium-dependent fluorescence of GCaMP6f excited by a 465 nm LED. D. Histological image of Cre-dependent GCaMP6f-expressing neurons in the striatum of a Calb1-IRES-Cre mouse. Scale bar: 500 µm. E. Schematic illustration of the measurement of GCaMP fluorescence from striosome neurons. GCaMP6f was selectively expressed in striosome neurons via injection of AAV5.CAG.Flex.GCaMP6f into the DMS of Pdyn-IRES-Cre mice. F. Histological image of Cre-dependent GCaMP6f-expressing neurons in the striatum of a Pdyn-IRES-Cre mouse. GCaMP6f was mosaically expressed in the striatum. Scale bar: 500 µm.

Article Snippet: For fiber photometry recordings, AAV5.CAG.Flex.GCaMP6f.WPRE.SV40 (left hemisphere: five mice, right hemisphere: five mice, 100835-AAV5, Addgene, Watertown, MA, USA) was injected into the dorsomedial striatum (DMS) (AP: +0.5, ML: 1.75, DV: 2.85 mm from the brain surface, volume: 400 nL) using a microsyringe pump (Legato100, Kd Scientific, Holliston, MA, USA).

Techniques: Fluorescence, Injection, Expressing

Journal: Nature communications

Article Title: A hippocampus-accumbens code guides goal-directed appetitive behavior.

doi: 10.1038/s41467-024-47361-x

Figure Lengend Snippet: Fig. 4 | dHPC→NAc place fields are modulated by local cues and reward zone. a Heat maps of dHPC- (left) and dHPC→NAc (right) place cells’ average calcium events, ordered by place field location. White dashed lines: texture boundaries; yellow rectangle: reward zone. b Example place fields with edges near belt texture boundaries. Triangles mark start (no fill) and end (fill) points. Dashed black lines: texture boundaries. c, d Place field edges accumulate near texture boundary areas. Histograms of dHPC- (green) and dHPC→NAc (red) neurons’ place field start c and end d. Dotted line and shade: average and 95th CI of 1000× randomly shuffled place fields. Both dHPC- and dHPC→NAc place field start and end positions are significantly overrepresented at the 99.9th percentile (dotted black lines) compared to a ran- domly shuffled distribution (mean represented by horizontal black line). Start (χ²(1, 5332) = 5.136, P = 0.0234) and end positions (χ²(1, 5332) = 3.869, P = 0.0492) of dHPC→NAc place fields are also significantly overrepresented compared to the dHPC-

Article Snippet: Then, we used double-floxed inverse open reading frame (DIO) Cre-dependent mCherry under the human synapsin promoter in dHPC (AAV5-hSyn-DIO-mCherry, titer: 1.1 × 1013 vg/ml, Catalog# 50459-AAV5, Addgene,Watertown,USA).

Techniques:

Journal: Nature communications

Article Title: A hippocampus-accumbens code guides goal-directed appetitive behavior.

doi: 10.1038/s41467-024-47361-x

Figure Lengend Snippet: Fig. 6 | dHPC→NAc neurons are over-represented in lick-excited neurons. a, b Example imaging session and traces showing lick-excited and lick-inhibited neurons. a Behavioral traces and calcium activity of sample neurons #11 (lick- inhibited) and #118 (lick-excited). b Field of view showing spatial profiles of dHPC-

Article Snippet: Then, we used double-floxed inverse open reading frame (DIO) Cre-dependent mCherry under the human synapsin promoter in dHPC (AAV5-hSyn-DIO-mCherry, titer: 1.1 × 1013 vg/ml, Catalog# 50459-AAV5, Addgene,Watertown,USA).

Techniques: Imaging, Activity Assay